Nanjing Norman Biological Technology Co., Ltd
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Place of Origin: | Jiangsu, China (Mainland) |
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Thyroid FT4 Chemiluminescence Immunoassay Kit
Methodology
Chemiluminescence Immunoassay
Product Show
FT4 Free
Thyroxine (FT4) Kit (Chemiluminescence Immunoassay) is intended for in
vitro quantitative determination of free thyroxine (FT4) content in
human serum, based on NRM411 system.
Thyroxine, also called
3,5,3',5'-tetraiodothyronine, is a hormone synthesized and secreted by
thyroid, which together with triiodothyronine (T3), play an important
role in the regulation of human growth and development including
metabolic rate, cardiovascular function, bone metabolism, gonadal
function and nervous system. In blood circulation, 99.95% of T4
reversibly binds to carrier proteins such as thyroxine-binding globulin
(TBG), few albumins and prealbumins, and the rest T4 remains in a free
state. FT4 has metabolic activity, and is also the precursor of T3.
Clinical Significance In
hypothyroidism and hyperthyroidism patients, FT4 and thyroxine (T4)
concentrations normally paralleling change. The FT4 assay is usually
combined with the detection of FT4 and TSH for clinical diagnosis of
thyroid function disorder. FT4 assay is also suitable for the curative
effect monitoring of thyroid suppression therapy. The determination of
FT4 has the advantage of being independent of changes in the
concentrations and binding properties of these binding proteins;
additional determination of a binding parameter (T-uptake, TBG) is
therefore unnecessary.
Test Principle The
assay is a competitive immunoassay for quantitative determination of
FT4 concentration based on chemiluminescence immunoassay technology. The
Magnetic Microbeads Reagent contains protecting reagents and magnetic
microbeads that are coated with biotin labeled T4 antibody. The Antibody
Reagent contains the protecting reagents and acridinium ester labeled
BSA-T4 conjugate. Sample, calibrator or control is mixed with Magnetic
Microbeads Reagent and Antibody Reagent to form a sandwich complex.
After incubation and automatic magnetic separation conducted by the
instrument, trigger solutions are added to generate chemiluminescent
reaction and the relative light units (RLUs) are measured by a
photomultiplier. A proportional relationship presents between the
concentration of FT4 in the sample and the RLUs detected by the NRM411
system.
Packages 100 Tests/Box, 200 Tests/Box.
Storage and Stability Sealed: Stable until the expiry date when stored at 2-8ºC.
Opened:
Stable for 4 weeks for reagents. Stable for 1 week after
dissolved/opened for solid control material/liquid control material. To
ensure the kit's best performance, it is recommended to place opened
kits in the refrigerator if it's not going to be used on board during
the next 12 hours.
Do not use it beyond the expiration date.
Calibration and Traceability
Calibration
Each batch of kits contains specific calibration information, stored in the Calibration Card.
Traceability
The FT4 assay is traceable to a NIFDC standard reference material (lot No. 150551-201203).